ABSTRACT
A Taqman qRT-PCR method for detection of Nipah virus (NiV) N gene was established and applied to the detection and quantification of NiV samples. Primers and probe were designed based on the conserved region of NiV N gene, and a Taqman qRT-PCR detection method was established. The sensitivity, specificity, and repeatability of the method were analyzed, and the method was used for sample detection. The analytical detection limit of this Taqman qRT-PCR method established in this study was 102 copies/pL and allowed quantitation ranging from 1.0 x 109 to 1.0 x 102 copies /L. This method showed good specificity and repeatability. NiV Malaysia strain and Bangladesh strain could be effectively detected by the Taqman qRT- PCR. 50 it can be used for the detection and quantification of NiV samples.